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notes biology imaging

Brachiopod larva in the Nikon Small World 2021

This image of a brachiopod larva was selected in the Nikon Small World 2021 photomicrography competition!

screenshot 20210918 094027 brave1441489128638992960
Categories
imaging biology notes

A mitotic wave

A mitotic wave traveling through an early #Drosophila #embryo #FlyFriday

3D HisGap cleavage cover
Early syncytial embryo of the fly Drosophila melanogaster. Nuclei (blue) are dividing in a wave from posterior to anterior. Membrane components (white) are already organized around the nuclei. The image is a frame from a timelapse acquired under lightsheet microscopy and rendered in 3D.
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biology imaging notes

The blastopore of bryozoan embryos

This is a bryozoan embryo exhibiting its blastopore. These animals are discreet but ubiquitous in oceans and lakes all over the world.

Bryozoan embryo during gastrulation revealing its blastopore.
Embryo of the bryozoan Membranipora membranacea under confocal microscopy.

What we see is the DNA inside the nucleus of the cells of the embryo. The color gradient indicates if the nuclei are closer (yellow) or further away (purple) from the microscope camera.

The embryonic cells are arranged in a circle and form a central opening that we call the blastopore. This opening, in bryozoans, will become the mouth of the animal after the embryo develops.

You can follow the process on video or learn more details in the paper.

What about our mouth, where does it come from?

Categories
biology imaging notes

Chubby ribbon worm

A chubby ribbon worm juvenile #Nemertean #WormWednesday

Chubby ribbon worm
Juvenile specimen of the nemertean Lineus ruber under wide field fluorescence microscopy. Magenta: Nuclei; Green: F-actin.

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biology imaging notes

Larva of a lamp shell

Larva of a lamp shell, also known as brachiopod.
Terebratalia transversa (Sowerby, 1846)
Oil on canvas
167.95 µm × 167.95 µm
Categories
biology imaging notes

Fruit fly embryo under lightsheet microscopy

A short video that I made about the embryonic development of the likeable Drosophila, also known as fruit fly or vinegar fly, won an honorable mention in the Small World in Motion.

A single embryo imaged from four different angles.

The details on the techniques I used and the video on its full resolution are available for download and re-use on the Wikimedia Commons.

Categories
imaging biology notes

The surface of a brachiopod embryo

Brachiopod embryo showing its surface and blastopore.
Embryo of the brachiopod Novocrania anomala at the gastrula stage showing its outer surface and the blastopore at the bottom. Cell membranes were stained (F-actin) and the original image stack was converted to a 3D animation using Fiji/ImageJ.
Categories
imaging notes

A typical lightsheet microscopy session

Here is how a typical session of imaging embryos under Lightsheet Microscopy goes. A glimpse into my day-to-day work :)

Assemble the incubation chamber:

Collect and mount the embryos:

Typical lightsheet microscopy session.

Acquire a short timelapse from multiple angles:

Transfer (lots of) data for image processing ;)

File transfer
Categories
imaging biology notes

Zebrafish laser eyes

Zebrafish embryo with laser eyes! A transgenic line for the gene Prox1 (orange) imaged with @zeiss_micro #embryo2017

Zebrafish laser eyes
Categories
biology imaging notes

Bryozoan embryos viewed from the animal and vegetal poles

The first 24 hours in 1.5 minutes of bryozoan embryos.